Relationship between interferon production and interferon messenger RNA synthesis in human fibroblasts.
نویسندگان
چکیده
منابع مشابه
Relationship between interferon production and interferon messenger RNA synthesis in human fibroblasts.
Poly(A) containing mRNA prepared from poly(rI)-poly(rC)-induced human fibroblasts stimulated [14C]leucine incorporation into protein in wheat germ cell-free extracts. For the translation of interferon mRNA into a biologically active product, the presence of spermine was essential. The protein synthesized in vitro fulfilled the criteria for human interferon--namely, its antiviral activity was sp...
متن کاملInterferon priming. Effects on interferon messenger RNA.
The effects of priming mouse cells with interferon on the production of interferon and its mRNA were investigated. Interferon-treated (primed) mouse L929 cells produce 3 to 10 times more interferon than do nonprimed cells following induction with Newcastle disease virus. Interferon appears 2 to 4 h sooner in the primed cultures than in nonprimed cultures and interferon production by primed cell...
متن کاملInterferon messenger RNA: translation in heterologous cells.
A viral inhibitor with the characteristics of mouse interferon is produced by avian and simian cells preincubated with RNA extracted from interferon-producing mouse cells. Similarly, RNA extracted from interferon-producing monkey cells induces a monkey interferon-like substance in avian cells and also in a line of simian cells, VERO, which normally lacks the capacity to produce its own interfer...
متن کاملReversible inhibition of interferon synthesis by puromycin: evidence for an interferon-specific messenger RNA.
19 Brock, T. D., Science, 129, 960 (1959). 20 Clamp, J. R., and L. Hough, Biochem. J., 94, 17 (1965). 21 Vidaver, A. M. K., Ph.D. dissertation, Indiana University, Bloomington (1965). 22 Stahl, E., and U. Kaltenbach, J. Chromatog., 5, 351 (1961). 23Falcone, G., and W. J. Nickerson, Science, 124, 272 (1956). 24 Nickerson, W. J., and G. Falcone, Science, 124, 318 (1956). 25Ibid., p. 722. 26 Lampe...
متن کاملInterferon activity produced by translation of human interferon messenger RNA in cell-free ribosomal systems and in Xenopus oöcytes.
Translation of messenger RNA isolated from poly(rI)-poly(rC)-induced human fibroblasts in cell-free ribosomal systems and in Xenopus oocytes resulted in the production of biologically active proteins that had the properties of human fibroblast interferon. The translation in the oocytes was much more efficient, giving approximately 500 times higher titers of interferon activity than the cell-fre...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1977
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.74.4.1483